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Energy-protein management by some warmwater finfishes. ArchiMer
Luguet, P; Moreau, Y.
If warmwater finfish protein requirements as level in diet is low, absolute intake per day is similar to those of coldwater species. But relation of this requirement with specific growth rate differs for strictly warmwater fishes as Tilapias. Analysis of partition between protein and non-protein energy retention shows that these species with Clarias make a better use of non-protein energy provided and consequently improve their protein retention. Better carbohydrate digestibility and metabolism are propounded as part of the explanation.
Tipo: Text Palavras-chave: Clarias; Oreochromis mossambicus; Oreochromis aureus; Oreochromis niloticus; Tilapia; Cichlidae; Pisces; Nutritional requirements; Digestibility; Diets; Proteins; Metabolism; Carbohydrates; Nutrition; Feeding; Growth rate.
Ano: 1989 URL: http://archimer.ifremer.fr/doc/1989/acte-1424.pdf
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Essential Fatty Acid Assimilation and Synthesis in Larvae of the Bivalve Crassostrea gigas ArchiMer
Da Costa Gonzalez, Fiz; Robert, Rene; Quere, Claudie; Wikfors, Gary H.; Soudant, Philippe.
Essential fatty acids (EFA) are important for bivalve larval survival and growth. The purpose of this study was to quantitatively assess for the first time through a mass-balance approach dietary EFA incorporation and synthesis within Crassostrea gigas larvae. A first experiment was carried out using two microalgae, Tisochrysis lutea (T) and Chaetoceros neogracile (Cg), as mono- and bi-specific diets. A second experiment using a similar design was performed to confirm and extend the results obtained in the first. Flow-through larval rearing was used for accurate control of food supply and measurement of ingestion. Non-methylene-interrupted fatty acids were synthetized from precursors supplied in the diet: 16:1n-7 and 18:1n-9, mediated by Delta 5...
Tipo: Text Palavras-chave: Fatty acid; Larvae; Lipids; Metabolism; Oyster; Synthesis.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00268/37962/36086.pdf
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Etude cytologique ultrastructurale des anomalies hepatiques du loup, de la daurade, et de l'anguille, induites par une alimentation artificielle ArchiMer
Bac, N; Biagianti, S; Brusle, J.
In the three species Dicentrarchus labrax, Sparus auratus , and Anguilla anguilla an artificial feeding (dry pellets) disturbs liver cells as revealed at ultrastructural level. In hepatocytes, anomalies consist of lipids storage (liposomes) considered as deviation in fatty acids metabolism. Moreover are observed cellular alterations (mitochondrial vacuolization, nuclear pycnosis) related to degenerative process. It is of great interest to understand wether liver modifications are just a reaction to an unbalanced feeding or a true trophic pathology.
Tipo: Text Palavras-chave: Dicentrarchus labrax; Serranidae; Pisces; Natural populations; Fish culture; Lipids; Fatty acids; Cytology; Ultrastructure; Cells; Diseases; Pellet feeds; Metabolism; Liver; Artificial feeding; Nutrition disorders.
Ano: 1983 URL: http://archimer.ifremer.fr/doc/1983/acte-1250.pdf
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Etude de la composition corporelle de la sole (Solea solea ) au cours du jeune: Influence de la temperature ArchiMer
Richard, P; Djabali, M; Lagardere, Francoise; Lagardere, J.
The effects of starvation on Solea solea at 10 and 20 degree C in sea water, were studied. At 20 degree C fishes start to die on week 4; at 10 degree C they support a longer starvation period (9 weeks). Proximate analysis shows that protein are more steadily used at 20 degree C than at 10 degree C, contrarily to the corporal lipids which are used at the same rate. Starvation results in a reduction of liver lipids and glycogen. This reduction is more pronounced at 20 degree C : 70% of hepatic glycogen is used at this temperature within the first week of starvation against 30% at 10 degree C.
Tipo: Text Palavras-chave: Solea solea; Soleidae; Pisces; Survival; Tissues; Biochemical composition; Temperature effects; Proteins; Lipids; Starvation; Metabolism.
Ano: 1983 URL: http://archimer.ifremer.fr/doc/1983/acte-1246.pdf
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Etude de la diversité métabolique des micro-organismes des sources hydrothermales ArchiMer
Byrne, Nathalie.
Since the discovery of oceanic hydrothermal vents, microbial diversity of this extreme environment was studied using both cultivation and molecular approaches. In this study the microbial diversity of active chimney from both TAG and Lucky Strike sites (Mid Atlantic Ridge) was investigated using both molecular survey and continuous enrichment culture in a bioreactor. To reproduce as close as possible environmental conditions the cultures were performed in oligotrophic conditions, in the presence of hydrogen and carbondioxide in the gas sweeping mix or using in situ sampled fluid/sea water mix as culture medium. The effect of environmental changes such as temperature and the absence or presence of sulphur was also investigated. Archaeal and Bacterial...
Tipo: Text Palavras-chave: Culture; Molecular diversity; Metabolism; Hydrothermal vents; Microorganisms; Microbiology; Culture; Diversité moléculaire; Métabolisme; Sources hydrothermales; Micro organismes; Microbiologie.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/these-7295.pdf
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Etude des liens trophiques au sein d'une communaute bacterienne selectionnee a partir d'un sediment marin sur le cyclohexene-4 dicarboximide-1,2 (THPI) ArchiMer
Terrom, G; Gil, G; Lepetit, J.
A bacterial community has been selected from a marine sediment on 4-cyclohexene-1,2 dicarboximide or THPI (fungicide). This community consists of nine strains. The "principal" strain, Photobacterium mandapamensis , degrades THPI by different metabolic pathways many of which require co-oxidative sequences. The other eight "auxiliary" strains occur at different states along the trophic chain formed by the "principal" strain, so increasing velocity and intensity of the THPI biodegradation.
Tipo: Text Palavras-chave: Bacteria; Marine pollution; Microorganisms; Metabolism; Fungicides; Biodegradation; Sediment pollution; Trophic relationships.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-1021.pdf
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Étude des voies de bioactivation du benzo[a]pyrène et du fluoranthène chez la sole commune (Solea solea) : Profil métabolique et génotoxicité ArchiMer
Wessel, Nathalie.
The aim of this Ph‟D was to study the biotransformation and the genotoxicity of two PAH of interest, namely the benzo[a]pyrene (BaP) and the fluoranthene (Fluo), in the common sole Solea solea, which is a benthic species sensitive to chemical pollution. The use of in vitro exposure models and the application of two different chemical analytical techniques allowed demonstrating that, as in higher vertebrates, the dihydrodiols and the phenols of BaP and Fluo are the major metabolites produced by sole liver enzymes. In vitro, the genotoxic potential of BaP and Fluo was demonstrated in sole, by the production of DNA adducts and oxidative DNA damage. The genotoxicity of Fluo appeared to be 50 fold less important than the genotoxicity of BaP. In the case of BaP,...
Tipo: Text Palavras-chave: Écotoxicologie; Hydrocarbures Aromatiques Polycycliques; Solea solea; Métabolisme; Génotoxicité; Ecotoxicology; Polycyclic Aromatic Hydrocarbons; Solea solea; Metabolism; Genotoxicity.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00032/14326/11609.pdf
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Etude experimentale preliminaire sur les echanges gazeux chez Pinna nobilis ArchiMer
Vicente, N; Riva, A; Butler, A.
Breathing exchanges of Pinna nobilis L. are studied in situ and in the laboratory with two methods. First method consist in introducing samples of Pinna under a 40l plexiglass bell equipped with haps. The bell is positionned by diving. The consumption of oxygen is observed with water samples and the oxygen concentration is measured with an electronic oxymeter. Experiments which are carried out during nycthemeral cycles are reproduced in the laboratory with the same type of bell. A second method carry into effect an apparatus more sophisticated, who put into circulation a current of water in an airtight thermostated cellule, where a specimen of young Pinna is introduced. This sample is gradually adaptated to the conditions of the medium. Breathing...
Tipo: Text Palavras-chave: Pinna nobilis; Bivalvia; Temperature; Metabolism; Oxygen consumption; Experimental research; Breathing mixtures; Marine molluscs.
Ano: 1991 URL: http://archimer.ifremer.fr/doc/1991/acte-1761.pdf
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Evaluation of blood metabolites in dairy cows grazing under two pasture allowances and supplemented with corn silage under restricted grazing conditions R. Bras. Zootec.
Morales,Álvaro; Grob,Daniela; Wittwer,Fernando; Müller,Ananda; Balocchi,Óscar; Pulido,Rubén.
ABSTRACT This study was conducted to evaluate the influence of management tools - daily pasture allowance and corn silage supplementation - during periods of forage shortage on the metabolism of dairy cows grazing low-mass pasture in temperate regions. Forty lactating Holstein cows were used during an experimental period of seventy days (April 15 to June 23, 2012). Blood metabolites and milk production were determined in fall-calving dairy cows grazing under two daily pasture allowances (PA) (moderate, 17 kg vs. high, 25 kg dry matter (DM)) and supplemented with corn silage (CS) (low, 4.5 kg vs. high, 9.0 kg DM). All cows received 3 kg DM of concentrate. Plasma concentrations of β-hydroxybutyrate (BHBA), non-esterified fatty acids (NEFA), and urea were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dairy cow; Grazing system; Metabolism; Restricted feeding; Supplementation.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982016001100686
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Experimental shift in diet Delta13C: A potential tool for ecophysiological studies in marine bivalves ArchiMer
Paulet, Yves-marie; Lorrain, Anne; Richard, Joëlle; Pouvreau, Stephane.
To test the potential of diet switching experiments in ecophysiological studies of marine invertebrates, stable carbon isotope ratios were measured at different seasons in the gonad, adductor muscle, digestive gland and gills of scallops (Pecten maximus) and oysters (Crassostrea gigas) held for 15 days on a constant diet of phytoplankton depleted in 13C. The aim of this study was to determine if differences in carbon incorporation could be detected among species, seasons and organs, and if so, whether it was consistent with their known energy-allocation patterns. After offering the new diet, isotope values of the different organs gradually shifted and significant differences among organs, seasons and species were found. A carbon incorporation index (CII)...
Tipo: Text Palavras-chave: Crassostrea gigas; Pecten maximus; Carbon isotopes; Metabolism; Energy allocation.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1202.pdf
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Expression of candidate genes related to metabolism, immunity and cellular stress during massive mortality in the American oyster Crassostrea virginica larvae in relation to biochemical and physiological parameters ArchiMer
Genard, Bertrand; Moraga, Dario; Pernet, Fabrice; David, Elise; Boudry, Pierre; Tremblay, Rejean.
Quantification of mRNA of genes related to metabolism, immunity and cellular stress was examined in relation to a massive mortality event during the culture of American oyster larvae, Crassostrea virginica which was probably, in regard to previous microbiological analysis, induced by vibrio infection. To document molecular changes associated with the mortality event, mRNA levels were compared to biochemical and physiological data, previously described in a companion paper. Among the 18 genes studied, comparatively to the antibiotic control, 10 showed a lower relative gene expression when the massive mortality occurred. Six of them are presumed to be related to metabolism, corroborating the metabolic depression associated with the mortality event suggested...
Tipo: Text Palavras-chave: Massive mortality; Relative gene expression; Metabolism; Immunity; Cellular stress; Larvae.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00075/18585/16138.pdf
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Farelo da vagem de algaroba associado a níveis de ureia na alimentação de ovinos: balanço de nitrogênio, N-ureico no plasma e parâmetros ruminais - doi: 10.4025/actascianimsci.v34i3.12759 Animal Sciences
Alves, Evanilton Moura; Instituto Federal Baiano; Pedreira, Márcio dos Santos; Estadual do Sudoeste da Bahia; Pereira, Mara Lúcia Albuquerque; Estadual do Sudoeste da Bahia; Almeida, Paulo José Presidio; Estadual do Sudoeste da Bahia; Gonsalves Neto, João; Estadual do Sudoeste da Bahia; Freire, Leile Daiane Ribeiro; Estadual do Sudoeste da Bahia.
Objetivou-se neste trabalho avaliar os efeitos da inclusão de ureia em dietas contendo farelo da vagem de algaroba sobre o balanço de nitrogênio, N-uréico no plasma e parâmetros ruminais em ovinos. Foram utilizados oito animais, machos castrados, com peso médio de 33,5 kg, distribuídos em dois quadrados latinos 4 x 4. Os tratamentos constituíram da inclusão de níveis de ureia na dieta, sendo: 0; 0,5; 1,0 e 1,5% da MS total. A dieta foi fornecida como dieta total na relação volumoso:concentrado de 40:60 com base na MS total da dieta. Foi realizada a coleta total de urina e fezes e determinada a excreção de nitrogênio. Foi coletado sangue, seguido com a extração do plasma e determinação do N-uréico. Para avaliação do pH e N-amoniacal no líquido ruminal foram...
Tipo: Pesquisa de campo; análise laboratorial Palavras-chave: Alimento alternativo; Confinamento; Metabolismo; Nitrogênio não protéico; Pequenos ruminantes alternative feed; Confinement; Metabolism; Non-proteic nitrogen; Small ruminants.
Ano: 2012 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/12759
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Feeding high-moisture corn grain silage to broilers fed alternative diets and maintained at different environmental temperatures Rev. Bras. Ciênc. Avic.
Cruz-Polycarpo,VC; Sartori,JR; Gonçalves,JC; Pinheiro,DF; Madeira,LA; Polycarpo,GV; Zanetti,LH; Santos,TS; Pezzato,AC.
The effects of the dietary substitution of dry corn by high-moisture corn grain silage (HMCGS) were evaluated on the performance, nutrient digestibility and serum biochemical parameters of broilers reared in an alternative production system and submitted to different environmental temperatures. A total of 288 one-day-old male Cobb chicks were distributed according to a randomized block design in a 3x4 factorial arrangement: three environmental temperatures (hot, thermoneutral or cold) and four levels of HMCGS in substitution of dry corn (0%, 20%, 40% or 60%). The acid analysis showed that the evaluated HMCGS contained average percentage values of ethanol, lactic acid, and acetic acid (expressed in 100% of dry matter) of 0.7690, 2.7320 and 0.0249%,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Absorption; Alternative feeds; Poultry; Cholesterol; Metabolism; Triglycerides.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2014000400017
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Foliar carbohydrates content and invertase activity in vines at São Francisco River Valley - Brazil Rev. Bras. Frutic.
Dantas,Barbara França; Ribeiro,Luciana de Sá; Silva,Alexandro Pereira da; Luz,Sara Raquel de Souza.
The irrigated agriculture at the São Francisco River Valley, Northeast Brazil, shows an increasing production of grapes for winery. Among the wines produced there the one obtained from Vitis vinifera L., cultivar Syrah, stands out due to its adaptation to the climatic conditions of the region. However, little is known about carbohydrates metabolism of vines cultivated in this region. The objective of this work was to evaluate sugar and starch contents and the invertase activity in vines leaves during two consecutive growing seasons. The experiment was carried out at Embrapa Semi-Árido and at Santa Maria Winery, respectively located in Petrolina and Lagoa Grande, Pernambuco-Brazil. Leaves were collected weekly from January to December of 2003 and assessed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Grape; Metabolism; Sugar.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452005000200004
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Gender-dependent association of HSD11B1 single nucleotide polymorphisms with glucose and HDL-C levels Genet. Mol. Biol.
Turek,Luciane Viater; Leite,Neiva; Souza,Ricardo Lehtonen Rodrigues; Lima,Jovana Karoline; Milano,Gerusa Eisfeld; Timossi,Luciana da Silva; Osiecki,Ana Claudia Vecchi; Osiecki,Raul; Alle,Lupe Furtado.
In this study, we investigated the influence of two SNPs (rs846910 and rs12086634) of the HSD11B1 gene that encodes 11β-hydroxysteroid dehydrogenase type 1(11β-HSD1), the enzyme that catalyzes the conversion of cortisol to cortisone, on variables associated with obesity and metabolic syndrome in 215 individuals of both sexes from southern Brazil. The HSD11B1 gene variants were genotyped using the TaqMan SNP genotyping assay. Glucose, triglycerides, total cholesterol, HDL-cholesterol and LDL-cholesterol were measured by standard automated methods. Significant results were found in women, with carriers of the G allele of SNP rs12086634 having higher glucose levels than non-carriers. Carriers of the A allele of SNP rs846910 had higher levels of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: HSD11B1 gene; Men; Metabolism; Metabolic syndrome; Women.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400003
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Genome-wide comparison of genes involved in the biosynthesis, metabolism, and signaling of juvenile hormone between silkworm and other insects Genet. Mol. Biol.
Cheng,Daojun; Meng,Meng; Peng,Jian; Qian,Wenliang; Kang,Lixia; Xia,Qingyou.
Juvenile hormone (JH) contributes to the regulation of larval molting and metamorphosis in insects. Herein, we comprehensively identified 55 genes involved in JH biosynthesis, metabolism and signaling in the silkworm (Bombyx mori) as well as 35 in Drosophila melanogaster, 35 in Anopheles gambiae, 36 in Apis mellifera, 47 in Tribolium castaneum, and 44 in Danaus plexippus. Comparative analysis showed that each gene involved in the early steps of the mevalonate (MVA) pathway, in the neuropeptide regulation of JH biosynthesis, or in JH signaling is a single copy in B. mori and other surveyed insects, indicating that these JH-related pathways or steps are likely conserved in all surveyed insects. However, each gene participating in the isoprenoid branch of JH...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Juvenile hormone; Biosynthesis; Metabolism; Signaling; Gene duplication.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300018
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Glutamine and glutamate as vital metabolites BJMBR
Newsholme,P.; Lima,M.M.R.; Procopio,J.; Pithon-Curi,T.C.; Doi,S.Q.; Bazotte,R.B.; Curi,R..
Glucose is widely accepted as the primary nutrient for the maintenance and promotion of cell function. This metabolite leads to production of ATP, NADPH and precursors for the synthesis of macromolecules such as nucleic acids and phospholipids. We propose that, in addition to glucose, the 5-carbon amino acids glutamine and glutamate should be considered to be equally important for maintenance and promotion of cell function. The functions of glutamine/glutamate are many, i.e., they are substrates for protein synthesis, anabolic precursors for muscle growth, they regulate acid-base balance in the kidney, they are substrates for ureagenesis in the liver and for hepatic and renal gluconeogenesis, they act as an oxidative fuel for the intestine and cells of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glutamine; Glutamate; Glucose; Metabolism; Cell function.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000200002
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Glycolysis and gluconeogenesis in the liver of catfish fed with different concentrations of proteins, lipids and carbohydrates Arq. Bras. Med. Vet. Zootec.
Melo,J.F.B.; Lundstedt,L. M; Inoue,L.A.K.; Metón,I.; Baanante,I.V.; Moraes,G..
ABSTRACT The activities of enzymes from a number of metabolic pathways have been used as a tool to evaluate the best use of nutrients on fish performance. In the present study the catfish Rhamdia quelen was fed with diets containing crude protein-lipid-carbohydrate (%) as follows: treatment (T) T1: 19-19-44; T2: 26-15-39; T3: 33-12-33; and T4: 40-10-24. The fish were held in tanks of re-circulated, filtered water with controlled temperature and aeration in 2000L experimental units. The feeding experiment lasted 30 days. The following enzymes of the carbohydrate metabolism were determined: Glucokinase (GK), Phosphofructokinase 1 (PFK-1), Pyruvate kinase (PK), Fructose-1,6-biphosphatase 1 (FBP-1). The activities of 6 phosphogluconate dehydrogenase (6PGDH)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Metabolism; Enzymes; Nutrition; Adaptation.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000501251
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Growth of Burkholderia sacchari LFM 101 cultivated in glucose, sucrose and glycerol at different temperatures Scientia Agricola
Nascimento,Valkirea Matos; Silva,Luiziana Ferreira; Gomez,José Gregório Cabrera; Fonseca,Gustavo Graciano.
ABSTRACT Polyhydroxyalkanoates (PHAs) have attracted major industrial interest as alternatives to conventional plastics. They are produced by several bacteria as cytoplasmic inclusions when nutrients are in limited supply. Among the many factors influencing bacterial growth, the effect of temperature on both specific growth rates and growth yields in terms of carbon source intake is of considerable interest. This study aimed to evaluate the influence of the bacterium Burkholderia sacchari LFM 101 on growth and PHA production, using glucose, sucrose or glycerol as a carbon source, at 30 and 35 °C. The results showed that B. sacchari cultured with glucose at 35 °C presented both higher productivity and polymer yield in dried cell mass. There were no...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Polyhydroxyalkanoates (PHAs); Metabolism; Kinetics.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162016000500429
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Growth performance and metabolic response of Nile tilapia fed rations supplemented with autolized yeast and zinc R. Bras. Zootec.
Signor,Altevir; Pezzato,Luiz Edivaldo; Padilha,Pedro de Magalhães; Padovani,Carlos Roberto; Barros,Margarida Maria.
This research evaluated the influence of yeast and zinc on growth performance and metabolic responses of Nile tilapia. Diets were formulated to contain 32.0% digestible protein and 3,240 kcal DE/kg diet and the following autolized yeast (%):zinc (mg/kg)relationships: 0.0:0.0; 0.0:79.5; 2.0:0.0; 0.795:79.5; 2.0:200; 4.0:400; 6.0:600; 12.0:1,200 and 14.0:1,400. It was used 135 fingerlings (7.27 ± 0.19 g), distributed in a complete random design in 27 50-L aquaria and they were fed ad libitum four times a day for 128 days. It was evaluated growth performance and metabolic responses, weight gain, apparent feed conversion; protein efficiency and survival rate; percentage of protein, ether extract, dry weight and ashes in the muscle and in the bones; ammonia...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fingerlings; Growth; Metabolism; Metabolites; Minerals.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010001200002
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